4asu

X-ray diffraction
2.6Å resolution

F1-ATPase in which all three catalytic sites contain bound nucleotide, with magnesium ion released in the Empty site

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-133621 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
ATP synthase subunit alpha, mitochondrial Chains: A, B, C
ATP synthase subunit beta, mitochondrial Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 480 amino acids
Theoretical weight: 51.62 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00829 (Residues: 49-528; Coverage: 91%)
Gene names: ATP5B, ATP5F1B
Sequence domains:
Structure domains:
ATP synthase subunit gamma, mitochondrial Chain: G
Molecule details ›
Chain: G
Length: 273 amino acids
Theoretical weight: 30.3 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05631 (Residues: 26-298; Coverage: 92%)
Gene names: ATP5C, ATP5C1, ATP5F1C
Sequence domains: ATP synthase
Structure domains:
ATP synthase subunit delta, mitochondrial Chain: H
Molecule details ›
Chain: H
Length: 146 amino acids
Theoretical weight: 15.07 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05630 (Residues: 23-168; Coverage: 87%)
Gene names: ATP5D, ATP5F1D
Sequence domains:
Structure domains: F0F1 ATP synthase delta/epsilon subunit, N-terminal
ATP synthase subunit epsilon, mitochondrial Chain: I
Molecule details ›
Chain: I
Length: 50 amino acids
Theoretical weight: 5.66 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P05632 (Residues: 2-51; Coverage: 98%)
Gene names: ATP5E, ATP5F1E
Sequence domains: Mitochondrial ATP synthase epsilon chain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 105.268Å b: 135.113Å c: 266.355Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.245 0.289