› 4awb

X-ray diffraction
2.7Å resolution

Crystal structure of active legumain in complex with AAN-CMK

Released:
DOI: 10.2210/pdb4awb/pdb
PDB entry 4awb coloured by chain, front view.
PDB entry 4awb coloured by chain, side view.
PDB entry 4awb coloured by chain, top view.
Source organisms:
Primary publication:
Mechanistic and structural studies on legumain explain its zymogenicity, distinct activation pathways, and regulation.
Dall E, Brandstetter H
Proc Natl Acad Sci U S A 110 10940-5 (2013)
PMID: 23776206

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164296 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Legumain Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 32.27 KDa
Source organism: Homo sapiens
Expression system: Leishmania tarentolae
UniProt:
  • Canonical: Q99538 (Residues: 26-309; Coverage: 68%)
Gene names: LGMN, PRSC1
Sequence domains: Peptidase C13 family
Structure domains: Rossmann fold
Z-ALA-ALA-AZAASN-CHLOROMETHYLKETONE Chains: I, J
Molecule details ›
Chains: I, J
Length: 5 amino acids
Theoretical weight: 460 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand NAG 6 x NAG
Ligand SO4 2 x SO4
Ligand HG 4 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41
Unit cell:
a: 63.9Å b: 63.9Å c: 144.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.229 0.255
Expression systems:
  • Leishmania tarentolae
  • Not provided

Quick links

Citations

19 review citations

Vacuolar processing enzyme in plant programmed cell death.
Hatsugai et al. (2015)
18 more

4 mentions without citation

Mechanisms Applied by Protein Inhibitors to Inhibit Cysteine Proteases.
TuĊĦar et al. (2021)
3 more