› 4ayd

X-ray diffraction
2.4Å resolution

Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 1 R106A mutant

Released:
DOI: 10.2210/pdb4ayd/pdb
PDB entry 4ayd coloured by chain, front view.
PDB entry 4ayd coloured by chain, side view.
PDB entry 4ayd coloured by chain, top view.
Source organisms:
Primary publication:
Design and evaluation of meningococcal vaccines through structure-based modification of host and pathogen molecules.
Johnson S, Tan L, van der Veen S, Caesar J, Goicoechea De Jorge E, Harding RJ, Bai X, Exley RM, Ward PN, Ruivo N, Trivedi K, Cumber E, Jones R, Newham L, Staunton D, Ufret-Vincenty R, Borrow R, Pickering MC, Lea SM, Tang CM
PLoS Pathog 8 e1002981 (2012)
PMID: 23133374

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140218 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Complement factor H Chains: A, B, E
Molecule details ›
Chains: A, B, E
Length: 125 amino acids
Theoretical weight: 14.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08603 (Residues: 321-443; Coverage: 10%)
Gene names: CFH, HF, HF1, HF2
Sequence domains: Sushi repeat (SCR repeat)
Structure domains: Complement Module, domain 1
Factor H binding protein Chains: C, D, F
Molecule details ›
Chains: C, D, F
Length: 257 amino acids
Theoretical weight: 27.61 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JXV4 (Residues: 27-274; Coverage: 97%)
Gene names: NMB1870, fhbP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 185.26Å b: 54.01Å c: 129.56Å
α: 90° β: 118.03° γ: 90°
R-values:
R R work R free
0.204 0.202 0.231
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structural vaccinology starts to deliver.
Dormitzer et al. (2012)
11 more

1 mention without citation

Structural characterization of a cross-protective natural chimera of factor H binding protein from meningococcal serogroup B strain NL096.
Veggi et al. (2022)