› 4ayi

X-ray diffraction
2.31Å resolution

Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 3 Wild type

Released:
DOI: 10.2210/pdb4ayi/pdb
PDB entry 4ayi coloured by chain, front view.
PDB entry 4ayi coloured by chain, side view.
PDB entry 4ayi coloured by chain, top view.
Source organisms:
Primary publication:
Design and evaluation of meningococcal vaccines through structure-based modification of host and pathogen molecules.
Johnson S, Tan L, van der Veen S, Caesar J, Goicoechea De Jorge E, Harding RJ, Bai X, Exley RM, Ward PN, Ruivo N, Trivedi K, Cumber E, Jones R, Newham L, Staunton D, Ufret-Vincenty R, Borrow R, Pickering MC, Lea SM, Tang CM
PLoS Pathog 8 e1002981 (2012)
PMID: 23133374

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-140216 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Complement factor H Chains: A, E
Molecule details ›
Chains: A, E
Length: 125 amino acids
Theoretical weight: 14.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli B
UniProt:
  • Canonical: P08603 (Residues: 321-443; Coverage: 10%)
Gene names: CFH, HF, HF1, HF2
Sequence domains: Sushi repeat (SCR repeat)
Structure domains: Complement Module, domain 1
Lipoprotein GNA1870 Chain: D
Molecule details ›
Chain: D
Length: 270 amino acids
Theoretical weight: 29.51 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli B
UniProt:
  • Canonical: Q19KF7 (Residues: 32-281; Coverage: 95%)
Gene name: gna1870
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41212
Unit cell:
a: 57.13Å b: 57.13Å c: 363.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.252
Expression system: Escherichia coli B

Quick links

Citations

12 review citations

Structural vaccinology starts to deliver.
Dormitzer et al. (2012)
11 more

6 mentions without citation

In silico Mapping of Protein Unfolding Mutations for Inherited Disease.
McCafferty et al. (2016)
5 more