› 4b2q

Electron Microscopy
37Å resolution

Model of the yeast F1Fo-ATP synthase dimer based on subtomogram average

Released:
DOI: 10.2210/pdb4b2q/pdb
PDB entry 4b2q coloured by chain, front view.
PDB entry 4b2q coloured by chain, side view.
PDB entry 4b2q coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the yeast F1Fo-ATP synthase dimer and its role in shaping the mitochondrial cristae.
Davies KM, Anselmi C, Wittig I, Faraldo-Gómez JD, Kühlbrandt W
Proc Natl Acad Sci U S A 109 13602-7 (2012)
PMID: 22864911
Related structures: EMD-2161

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 46-mer (preferred)
PDBe Complex ID:
PDB-CPX-133627 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
ATP synthase subunit alpha, mitochondrial Chains: A, C, a, c
Molecule details ›
Chains: A, C, a, c
Length: 485 amino acids
Theoretical weight: 52.38 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P07251 (Residues: 61-545; Coverage: 89%)
Gene names: ATP1, YBL0827, YBL099W
Sequence domains:
ATP synthase subunit alpha, mitochondrial Chains: B, b
Molecule details ›
Chains: B, b
Length: 486 amino acids
Theoretical weight: 52.45 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P07251 (Residues: 60-545; Coverage: 89%)
Gene names: ATP1, YBL0827, YBL099W
Sequence domains:
ATP synthase subunit beta, mitochondrial Chains: D, d
Molecule details ›
Chains: D, d
Length: 470 amino acids
Theoretical weight: 50.44 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00830 (Residues: 39-508; Coverage: 92%)
Gene names: ATP2, J2041, YJR121W
Sequence domains:
ATP synthase subunit beta, mitochondrial Chains: E, F, e, f
Molecule details ›
Chains: E, F, e, f
Length: 473 amino acids
Theoretical weight: 50.75 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00830 (Residues: 39-511; Coverage: 93%)
Gene names: ATP2, J2041, YJR121W
Sequence domains:
ATP synthase subunit gamma, mitochondrial Chains: G, g
Molecule details ›
Chains: G, g
Length: 278 amino acids
Theoretical weight: 30.66 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38077 (Residues: 34-311; Coverage: 89%)
Gene names: ATP3, ATP3a, ATP3b, YBR039W, YBR0408
Sequence domains: ATP synthase
ATP synthase subunit delta, mitochondrial Chains: H, h
Molecule details ›
Chains: H, h
Length: 132 amino acids
Theoretical weight: 14.08 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12165 (Residues: 29-160; Coverage: 83%)
Gene names: ATP16, D2935, YD8119.03, YDL004W
Sequence domains:
ATP synthase subunit epsilon, mitochondrial Chains: I, i
Molecule details ›
Chains: I, i
Length: 59 amino acids
Theoretical weight: 6.39 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P21306 (Residues: 2-60; Coverage: 95%)
Gene names: ATP15, P0345, YPL271W
Sequence domains: Mitochondrial ATP synthase epsilon chain
ATP synthase subunit 9, mitochondrial Chains: J, K, L, M, N, O, P, Q, R, S, j, k, l, m, n, o, p, q, r, s
Molecule details ›
Chains: J, K, L, M, N, O, P, Q, R, S, j, k, l, m, n, o, p, q, r, s
Length: 76 amino acids
Theoretical weight: 7.76 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P61829 (Residues: 1-76; Coverage: 100%)
Gene names: ATP9, OLI1, OLI3, PHO2, Q0130
Sequence domains: ATP synthase subunit C
ATP synthase F(0) complex subunit B1, mitochondrial Chains: T, t
Molecule details ›
Chains: T, t
Length: 129 amino acids
Theoretical weight: 15.41 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13619 (Residues: 121-249; Coverage: 50%)
Gene names: ATP5F, ATP5F1, ATP5PB
Sequence domains: Mitochondrial ATP synthase B chain precursor (ATP-synt_B)
ATP synthase subunit d, mitochondrial Chains: U, u
Molecule details ›
Chains: U, u
Length: 120 amino acids
Theoretical weight: 13.77 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13620 (Residues: 5-124; Coverage: 75%)
Gene names: ATP5H, ATP5PD
Sequence domains: ATP synthase D chain, mitochondrial (ATP5H)
ATP synthase-coupling factor 6, mitochondrial Chains: V, v
Molecule details ›
Chains: V, v
Length: 66 amino acids
Theoretical weight: 7.83 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02721 (Residues: 36-101; Coverage: 61%)
Gene names: ATP5J, ATP5PF
Sequence domains: Mitochondrial ATP synthase coupling factor 6
ATP synthase subunit O, mitochondrial Chains: W, w
Molecule details ›
Chains: W, w
Length: 120 amino acids
Theoretical weight: 13.24 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P13621 (Residues: 24-143; Coverage: 56%)
Gene names: ATP5O, ATP5PO
Sequence domains: ATP synthase delta (OSCP) subunit

Ligands and Environments

3 bound ligands:
Ligand ATP 6 x ATP
Ligand MG 10 x MG
Ligand ADP 4 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 37Å
Relevant EMDB volumes: EMD-2161

Quick links

Citations

82 review citations

Mitochondrial form and function.
Friedman et al. (2014)
81 more

5 mentions without citation

From ATP to PTP and Back: A Dual Function for the Mitochondrial ATP Synthase.
Bernardi et al. (2015)
4 more