› 4b3e

X-ray diffraction
2.15Å resolution

Structure of copper-zinc superoxide dismutase complexed with bicarbonate.

Released:
DOI: 10.2210/pdb4b3e/pdb
PDB entry 4b3e coloured by chain, front view.
PDB entry 4b3e coloured by chain, side view.
PDB entry 4b3e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural evidence for a copper-bound carbonate intermediate in the peroxidase and dismutase activities of superoxide dismutase.
Strange RW, Hough MA, Antonyuk SV, Hasnain SS
PLoS One 7 e44811 (2012)
PMID: 22984565

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Cu-Zn] Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 154 amino acids
Theoretical weight: 15.96 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00441 (Residues: 1-154; Coverage: 100%)
Gene name: SOD1
Sequence domains: Copper/zinc superoxide dismutase (SODC)
Structure domains: Superoxide dismutase, copper/zinc binding domain

Ligands and Environments

4 bound ligands:
Ligand CU 10 x CU
Ligand ZN 10 x ZN
Ligand CO3 10 x CO3
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: C2221
Unit cell:
a: 165.994Å b: 203.107Å c: 144.384Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.215
Expression system: Saccharomyces cerevisiae

Quick links

Citations

4 review citations

Superoxide dismutases and superoxide reductases.
Sheng et al. (2014)
3 more

4 mentions without citation

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB Signaling.
Malty et al. (2017)
3 more