Function and Biology Details
Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
Protease and RNA and DNA (preferred)
PDBe Complex ID:
PDB-CPX-117233 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (4 distinct):
Reverse transcriptase/ribonuclease H
Molecule details ›
Chain: A
Length: 560 amino acids
Theoretical weight: 64.38 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli M15
UniProt:
Sequence domains:
Length: 560 amino acids
Theoretical weight: 64.38 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli M15
UniProt:
- Canonical:
P04585 (Residues: 588-1147; Coverage: 39%)
Sequence domains:
p51 RT
Molecule details ›
Chain: B
Length: 454 amino acids
Theoretical weight: 52.97 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli M15
UniProt:
Sequence domains:
Length: 454 amino acids
Theoretical weight: 52.97 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli M15
UniProt:
- Canonical: P04585 (Residues: 588-1027; Coverage: 31%)
Sequence domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 22-BM
Spacegroup:
P6122
Expression system: Escherichia coli M15
