› 4b53

X-ray diffraction
1.8Å resolution

Crystal structure of the isolated IgG4 CH3 domain

Released:
DOI: 10.2210/pdb4b53/pdb
PDB entry 4b53 coloured by chain, front view.
PDB entry 4b53 coloured by chain, side view.
PDB entry 4b53 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human IgG4 C(H)3 dimer reveals the role of Arg409 in the mechanism of Fab-arm exchange.
Davies AM, Rispens T, den Bleker TH, McDonnell JM, Gould HJ, Aalberse RC, Sutton BJ
Mol Immunol 54 1-7 (2013)
PMID: 23164605

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin heavy constant gamma 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 12.06 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01861 (Residues: 222-325; Coverage: 26%)
Gene name: IGHG4
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

4 bound ligands:
Ligand EDO 5 x EDO
Ligand PEG 4 x PEG
Ligand TRS 1 x TRS
Ligand ACT 1 x ACT
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P4322
Unit cell:
a: 57.09Å b: 57.09Å c: 138.436Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.237
Expression system: Homo sapiens

Quick links

Citations

13 review citations

IgG4-related disease.
Mahajan et al. (2014)
12 more

5 mentions without citation

Insights into the IgG heavy chain engineering patent landscape as applied to IgG4 antibody development.
Dumet et al. (2019)
4 more