› 4bdv

X-ray diffraction
3.98Å resolution

CRYSTAL STRUCTURE OF A TRUNCATED B-DOMAIN HUMAN FACTOR VIII

Released:
DOI: 10.2210/pdb4bdv/pdb
PDB entry 4bdv coloured by chain, front view.
PDB entry 4bdv coloured by chain, side view.
PDB entry 4bdv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Evaluation of the metal binding sites in a recombinant coagulation factor VIII identifies two sites with unique metal binding properties.
Svensson LA, Thim L, Olsen OH, Nicolaisen EM
Biol Chem 394 761-5 (2013)
PMID: 23435097

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132519 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Factor VIIIa heavy chain, 92 kDa isoform Chain: A
Molecule details ›
Chain: A
Length: 760 amino acids
Theoretical weight: 87.11 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00451 (Residues: 20-769; Coverage: 32%)
Gene names: F8, F8C
Sequence domains: Multicopper oxidase
Structure domains: Cupredoxins - blue copper proteins
Factor VIIIa light chain Chain: B
Molecule details ›
Chain: B
Length: 685 amino acids
Theoretical weight: 79.24 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P00451 (Residues: 1667-2351; Coverage: 29%)
Gene names: F8, F8C
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
4 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 1 x CA
Ligand EDO 2 x EDO
Ligand CU1 1 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P41212
Unit cell:
a: 133.84Å b: 133.84Å c: 355.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.162 0.246
Expression system: Cricetulus griseus

Quick links

Citations

2 review citations

A new recombinant factor VIII: from genetics to clinical use.
Santagostino E. (2014)
1 more

9 mentions without citation

Turoctocog alfa (NovoEight®)--from design to clinical proof of concept.
Ezban et al. (2014)
8 more