› 4beh

Solution NMR

Solution structure of human ribosomal protein P1.P2 heterodimer

Released:
DOI: 10.2210/pdb4beh/pdb
PDB entry 4beh coloured by chain, ensemble of 20 models, front view.
PDB entry 4beh coloured by chain, ensemble of 20 models, side view.
PDB entry 4beh coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of human P1•P2 heterodimer provides insights into the role of eukaryotic stalk in recruiting the ribosome-inactivating protein trichosanthin to the ribosome.
Lee KM, Yusa K, Chu LO, Yu CW, Oono M, Miyoshi T, Ito K, Shaw PC, Wong KB, Uchiumi T
Nucleic Acids Res 41 8776-87 (2013)
PMID: 23892290

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138645 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Large ribosomal subunit protein P1 Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 11.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05386 (Residues: 1-114; Coverage: 100%)
Gene names: RPLP1, RRP1
Sequence domains: 60s Acidic ribosomal protein
Structure domains: Arc Repressor Mutant, subunit A
Large ribosomal subunit protein P2 Chain: B
Molecule details ›
Chain: B
Length: 116 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05387 (Residues: 1-115; Coverage: 100%)
Gene names: D11S2243E, RPLP2, RPP2
Sequence domains: 60s Acidic ribosomal protein
Structure domains: Arc Repressor Mutant, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 64%
Refinement method: ARIA
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Structures and Ribosomal Interaction of Ribosome-Inactivating Proteins.
Shi et al. (2016)
5 more

6 mentions without citation

Solution structure of human P1•P2 heterodimer provides insights into the role of eukaryotic stalk in recruiting the ribosome-inactivating protein trichosanthin to the ribosome.
Lee et al. (2013)
5 more