4bg6

X-ray diffraction
2.3Å resolution

14-3-3 interaction with Rnd3 prenyl-phosphorylation motif

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-158295 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 27.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63104 (Residues: 1-245; Coverage: 100%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Rho-related GTP-binding protein RhoE Chains: Q, R
Molecule details ›
Chains: Q, R
Length: 10 amino acids
Theoretical weight: 1.25 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P61587 (Residues: 232-241; Coverage: 4%)
Gene names: ARHE, RHO8, RHOE, RND3

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 70.783Å b: 81.313Å c: 111.189Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.244 0.273
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided