› 4bjo

X-ray diffraction
2.06Å resolution

Nitrate in the active site of PTP1b is a putative mimetic of the transition state

Released:
DOI: 10.2210/pdb4bjo/pdb
PDB entry 4bjo coloured by chain, front view.
PDB entry 4bjo coloured by chain, side view.
PDB entry 4bjo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Nitrate in the active site of protein tyrosine phosphatase 1B is a putative mimetic of the transition state.
Kenny PW, Newman J, Peat TS
Acta Crystallogr D Biol Crystallogr 70 565-71 (2014)
PMID: 24531490

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 338 amino acids
Theoretical weight: 39.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18031 (Residues: 2-321; Coverage: 74%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

4 bound ligands:
Ligand CL 4 x CL
Ligand MG 2 x MG
Ligand NO3 2 x NO3
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: C2
Unit cell:
a: 161.62Å b: 66.765Å c: 68.771Å
α: 90° β: 95.01° γ: 90°
R-values:
R R work R free
0.217 0.215 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

The interactions of metal cations and oxyanions with protein tyrosine phosphatase 1B.
Singh et al. (2017)

2 mentions without citation

The metal face of protein tyrosine phosphatase 1B.
Bellomo et al. (2016)
1 more