› 4bl5

X-ray diffraction
2.6Å resolution

Crystal structure of human GDP-L-fucose synthase with bound NADP and product GDP-L-fucose

Released:
DOI: 10.2210/pdb4bl5/pdb
PDB entry 4bl5 coloured by chain, front view.
PDB entry 4bl5 coloured by chain, side view.
PDB entry 4bl5 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Vollmar M, Shafqat N, Rojkova A, Krojer T, Bradley A, Raynor JW, Kavanagh K, von Delft F, Arrowsmith CH, Bountra C, Edwards A, Oppermann U, Yue WW

Function and Biology Details

Reaction catalysed: 
GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GDP-L-fucose synthase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 336 amino acids
Theoretical weight: 37.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13630 (Residues: 8-321; Coverage: 98%)
Gene names: GFUS, SDR4E1, TSTA3
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 12 x NAP
2 bound ligands:
Ligand GFB 12 x GFB
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 90.407Å b: 90.478Å c: 147.518Å
α: 72.54° β: 72.64° γ: 60.14°
R-values:
R R work R free
0.225 0.222 0.286
Expression system: Escherichia coli BL21(DE3)

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