› 4bm7

X-ray diffraction
1.95Å resolution

Crystal Structure of IgG Fc F241A mutant with native glycosylation

Released:
DOI: 10.2210/pdb4bm7/pdb
PDB entry 4bm7 coloured by chain, front view.
PDB entry 4bm7 coloured by chain, side view.
PDB entry 4bm7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineering hydrophobic protein-carbohydrate interactions to fine-tune monoclonal antibodies.
Yu X, Baruah K, Harvey DJ, Vasiljevic S, Alonzi DS, Song BD, Higgins MK, Bowden TA, Scanlan CN, Crispin M
J Am Chem Soc 135 9723-32 (2013)
PMID: 23745692

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134812 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.28 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 106-329; Coverage: 56%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUL
Carbohydrate polymer
1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 48.868Å b: 73.375Å c: 135.951Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.234
Expression system: Homo sapiens

Quick links

Citations

16 review citations

Differential antibody glycosylation in autoimmunity: sweet biomarker or modulator of disease activity?
Seeling et al. (2017)
15 more

5 mentions without citation

Structural characterization of anti-inflammatory immunoglobulin G Fc proteins.
Ahmed et al. (2014)
4 more