› 4bn2

X-ray diffraction
2.7Å resolution

The crystal structure of kinesin-like protein KIF15

Released:
DOI: 10.2210/pdb4bn2/pdb
PDB entry 4bn2 coloured by chain, front view.
PDB entry 4bn2 coloured by chain, side view.
PDB entry 4bn2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure and biochemical characterization of Kif15: a bifunctional molecular motor involved in bipolar spindle formation and neuronal development.
Klejnot M, Falnikar A, Ulaganathan V, Cross RA, Baas PW, Kozielski F
Acta Crystallogr D Biol Crystallogr 70 123-33 (2014)
PMID: 24419385

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF15 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 361 amino acids
Theoretical weight: 39.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NS87 (Residues: 19-375; Coverage: 26%)
Gene names: KIF15, KLP2, KNSL7
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

2 bound ligands:
Ligand ADP 3 x ADP
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3221
Unit cell:
a: 90.08Å b: 90.08Å c: 249.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.202 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Prime movers: the mechanochemistry of mitotic kinesins.
Cross et al. (2014)
2 more

5 mentions without citation

The crystal structure and biochemical characterization of Kif15: a bifunctional molecular motor involved in bipolar spindle formation and neuronal development.
Klejnot et al. (2014)
4 more