4bpi

X-ray diffraction
1.98Å resolution

Mcl-1 bound to alpha beta Puma BH3 peptide 2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-161279 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Induced myeloid leukemia cell differentiation protein Mcl-1; Induced myeloid leukemia cell differentiation protein Mcl-1 homolog Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.23 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P97287 (Residues: 152-221; Coverage: 21%)
  • Canonical: Q07820 (Residues: 241-327; Coverage: 25%)
Gene names: BCL2L3, MCL1, Mcl1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Blc2-like
Bcl-2-binding component 3, isoforms 1/2 Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.24 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9BXH1 (Residues: 134-152; Coverage: 10%)
Gene names: BBC3, PUMA

Ligands and Environments

1 bound ligand:

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P3221
Unit cell:
a: 53.78Å b: 53.78Å c: 93.965Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.217 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided