› 4bqw

X-ray diffraction
1.79Å resolution

HIF prolyl hydroxylase 2 (PHD2/ EGLN1) in complex with Mn(II) and 2-(4-hydroxy-2-oxo-1,2-dihydroquinoline-3-carboxamido)acetic acid

Released:
DOI: 10.2210/pdb4bqw/pdb
PDB entry 4bqw coloured by chain, front view.
PDB entry 4bqw coloured by chain, side view.
PDB entry 4bqw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Selective small molecule probes for the hypoxia inducible factor (HIF) prolyl hydroxylases.
Chowdhury R, Candela-Lena JI, Chan MC, Greenald DJ, Yeoh KK, Tian YM, McDonough MA, Tumber A, Rose NR, Conejo-Garcia A, Demetriades M, Mathavan S, Kawamura A, Lee MK, van Eeden F, Pugh CW, Ratcliffe PJ, Schofield CJ
ACS Chem Biol 8 1488-96 (2013)
PMID: 23683440

Function and Biology Details

Reaction catalysed: 
Hypoxia-inducible factor-L-proline + 2-oxoglutarate + O(2) = hypoxia-inducible factor-trans-4-hydroxy-L-proline + succinate + CO(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Egl nine homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 28.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9GZT9 (Residues: 181-426; Coverage: 58%)
Gene names: C1orf12, EGLN1, PNAS-118, PNAS-137
Sequence domains: 2OG-Fe(II) oxygenase superfamily
Structure domains: q2cbj1_9rhob like domain

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand QNM 1 x QNM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P63
Unit cell:
a: 109.914Å b: 109.914Å c: 39.409Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.195 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Hypoxia-Modified Cancer Cell Metabolism.
Al Tameemi et al. (2019)
10 more

1 mention without citation

Potent and Selective Triazole-Based Inhibitors of the Hypoxia-Inducible Factor Prolyl-Hydroxylases with Activity in the Murine Brain.
Chan et al. (2015)