› 4bwe

X-ray diffraction
2.46Å resolution

Crystal structure of C-terminally truncated glypican-1 after controlled dehydration to 86 percent relative humidity

Released:
DOI: 10.2210/pdb4bwe/pdb
PDB entry 4bwe coloured by chain, front view.
PDB entry 4bwe coloured by chain, side view.
PDB entry 4bwe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration.
Awad W, Svensson Birkedal G, Thunnissen MM, Mani K, Logan DT
Acta Crystallogr D Biol Crystallogr 69 2524-33 (2013)
PMID: 24311593

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152787 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Secreted glypican-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 478 amino acids
Theoretical weight: 53.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P35052 (Residues: 24-479; Coverage: 85%)
Gene name: GPC1
Sequence domains: Glypican

Ligands and Environments

2 bound ligands:
Ligand NAG 4 x NAG
Ligand CA 9 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21
Unit cell:
a: 47.23Å b: 166.72Å c: 139.09Å
α: 90° β: 90.8° γ: 90°
R-values:
R R work R free
0.231 0.229 0.28
Expression system: Homo sapiens

Quick links

Citations

4 citation in other articles

Dramatic improvement of crystals of large RNAs by cation replacement and dehydration.
Zhang et al. (2014)
3 more

4 mentions without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)
3 more