4c2a

X-ray diffraction
2.08Å resolution

Crystal Structure of High-Affinity von Willebrand Factor A1 domain with R1306Q and I1309V Mutations in Complex with High Affinity GPIb alpha

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-137564 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
von Willebrand factor Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 24.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04275 (Residues: 1264-1471; Coverage: 8%)
Gene names: F8VWF, VWF
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain
Glycocalicin Chain: B
Molecule details ›
Chain: B
Length: 291 amino acids
Theoretical weight: 32.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P07359 (Residues: 17-306; Coverage: 46%)
Gene name: GP1BA
Sequence domains:
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-BM-B
Spacegroup: P61
Unit cell:
a: 89.06Å b: 89.06Å c: 124.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.189
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens