› 4c2x

X-ray diffraction
2.33Å resolution

Human N-myristoyltransferase isoform 2 (NMT2)

Released:
DOI: 10.2210/pdb4c2x/pdb
PDB entry 4c2x coloured by chain, front view.
PDB entry 4c2x coloured by chain, side view.
PDB entry 4c2x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Global profiling of co- and post-translationally N-myristoylated proteomes in human cells.
Thinon E, Serwa RA, Broncel M, Brannigan JA, Brassat U, Wright MH, Heal WP, Wilkinson AJ, Mann DJ, Tate EW
Nat Commun 5 4919 (2014)
PMID: 25255805

Function and Biology Details

Reaction catalysed: 
Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130015 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycylpeptide N-tetradecanoyltransferase 2 Chain: A
Molecule details ›
Chain: A
Length: 410 amino acids
Theoretical weight: 47.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60551 (Residues: 112-498; Coverage: 78%)
Gene name: NMT2
Sequence domains:
Structure domains: Aminopeptidase

Ligands and Environments


Cofactor: Ligand NHW 1 x NHW
1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 49.7Å b: 72.32Å c: 114.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.218 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

34 review citations

Reading m6A in the Transcriptome: m6A-Binding Proteins.
Patil et al. (2018)
33 more

3 mentions without citation

Structure and Functional Diversity of GCN5-Related N-Acetyltransferases (GNAT).
Salah Ud-Din et al. (2016)
2 more