› 4c3r

X-ray diffraction
2.79Å resolution

Structure of dephosphorylated Aurora A (122-403) bound to AMPPCP

Released:
DOI: 10.2210/pdb4c3r/pdb
PDB entry 4c3r coloured by chain, front view.
PDB entry 4c3r coloured by chain, side view.
PDB entry 4c3r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular mechanism of Aurora A kinase autophosphorylation and its allosteric activation by TPX2.
Zorba A, Buosi V, Kutter S, Kern N, Pontiggia F, Cho YJ, Kern D
Elife 3 e02667 (2014)
PMID: 24867643

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127232 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aurora kinase A Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 32.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14965 (Residues: 122-403; Coverage: 70%)
Gene names: AIK, AIRK1, ARK1, AURA, AURKA, AYK1, BTAK, IAK1, STK15, STK6
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P6122
Unit cell:
a: 83.468Å b: 83.468Å c: 172.63Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.221 0.306
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

The functional diversity of Aurora kinases: a comprehensive review.
Willems et al. (2018)
24 more

5 mentions without citation

Molecular mechanism of Aurora A kinase autophosphorylation and its allosteric activation by TPX2.
Zorba et al. (2014)
4 more