› 4c58

X-ray diffraction
2.16Å resolution

Structure of GAK kinase in complex with nanobody (NbGAK_4)

Released:
DOI: 10.2210/pdb4c58/pdb
PDB entry 4c58 coloured by chain, front view.
PDB entry 4c58 coloured by chain, side view.
PDB entry 4c58 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of cyclin G-associated kinase (GAK) trapped in different conformations using nanobodies.
Chaikuad A, Keates T, Vincke C, Kaufholz M, Zenn M, Zimmermann B, Gutiérrez C, Zhang RG, Hatzos-Skintges C, Joachimiak A, Muyldermans S, Herberg FW, Knapp S, Müller S
Biochem J 459 59-69 (2014)
PMID: 24438162

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-127252 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-G-associated kinase Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 38.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14976 (Residues: 14-351; Coverage: 26%)
Gene names: DNAJC26, GAK
Sequence domains: Protein kinase domain
Structure domains:
nanobody Chain: B
Molecule details ›
Chain: B
Length: 140 amino acids
Theoretical weight: 15.09 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand 824 1 x 824
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 156.866Å b: 37.43Å c: 76.01Å
α: 90° β: 108.48° γ: 90°
R-values:
R R work R free
0.182 0.179 0.242
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

12 review citations

Nanobodies and recombinant binders in cell biology.
Helma et al. (2015)
11 more

4 mentions without citation

COVID-19: Drug Targets and Potential Treatments.
Gil et al. (2020)
3 more