› 4c8b

X-ray diffraction
2.75Å resolution

Structure of the kinase domain of human RIPK2 in complex with ponatinib

Released:
DOI: 10.2210/pdb4c8b/pdb
PDB entry 4c8b coloured by chain, front view.
PDB entry 4c8b coloured by chain, side view.
PDB entry 4c8b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inflammatory Signaling by NOD-RIPK2 Is Inhibited by Clinically Relevant Type II Kinase Inhibitors.
Canning P, Ruan Q, Schwerd T, Hrdinka M, Maki JL, Saleh D, Suebsuwong C, Ray S, Brennan PE, Cuny GD, Uhlig HH, Gyrd-Hansen M, Degterev A, Bullock AN
Chem Biol 22 1174-84 (2015)
PMID: 26320862

Function and Biology Details

Reactions catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Receptor-interacting serine/threonine-protein kinase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 38.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43353 (Residues: 8-317; Coverage: 57%)
Gene names: CARDIAK, RICK, RIP2, RIPK2, UNQ277/PRO314/PRO34092
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains: Transferase(Phosphotransferase) domain 1

Ligands and Environments

2 bound ligands:
Ligand 0LI 2 x 0LI
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 58.735Å b: 86.682Å c: 137.335Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.244
Expression system: Spodoptera frugiperda

Quick links

Citations

9 review citations

NOD Signaling and Cell Death.
Heim et al. (2019)
8 more

15 mentions without citation

Insights into the molecular basis of the NOD2 signalling pathway.
Boyle et al. (2014)
14 more