› 4c93

X-ray diffraction
2.69Å resolution

Crystal structure of the carboxy-terminal domain of yeast Ctf4 bound to Pol alpha.

Released:
DOI: 10.2210/pdb4c93/pdb
PDB entry 4c93 coloured by chain, front view.
PDB entry 4c93 coloured by chain, side view.
PDB entry 4c93 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome.
Simon AC, Zhou JC, Perera RL, van Deursen F, Evrin C, Ivanova ME, Kilkenny ML, Renault L, Kjaer S, Matak-Vinković D, Labib K, Costa A, Pellegrini L
Nature 510 293-297 (2014)
PMID: 24805245

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146557 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA polymerase alpha-binding protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 478 amino acids
Theoretical weight: 54.45 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01454 (Residues: 471-927; Coverage: 49%)
Gene names: CHL15, CTF4, P9659.7, POB1, YPR135W
Sequence domains:
DNA polymerase alpha catalytic subunit A Chains: D, E
Molecule details ›
Chains: D, E
Length: 13 amino acids
Theoretical weight: 1.51 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P13382 (Residues: 137-149; Coverage: 1%)
Gene names: CDC17, N2181, POL1, YNL102W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Unit cell:
a: 88.977Å b: 99.998Å c: 219.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.21
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

44 review citations

SMC complexes: from DNA to chromosomes.
Uhlmann F. (2016)
43 more

3 mentions without citation

A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome.
Simon et al. (2014)
2 more