› 4cdg

X-ray diffraction
2.79Å resolution

Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody

Released:
DOI: 10.2210/pdb4cdg/pdb
PDB entry 4cdg coloured by chain, front view.
PDB entry 4cdg coloured by chain, side view.
PDB entry 4cdg coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.
Newman JA, Savitsky P, Allerston CK, Bizard AH, Özer Ö, Sarlós K, Liu Y, Pardon E, Steyaert J, Hickson ID, Gileadi O
Nucleic Acids Res 43 5221-35 (2015)
PMID: 25901030

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211302 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RecQ-like DNA helicase BLM Chains: A, B
Molecule details ›
Chains: A, B
Length: 673 amino acids
Theoretical weight: 76.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54132 (Residues: 636-1298; Coverage: 47%)
Gene names: BLM, RECQ2, RECQL3
Sequence domains:
Structure domains:
NANOBODY Chains: C, D
Molecule details ›
Chains: C, D
Length: 147 amino acids
Theoretical weight: 16.17 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P1
Unit cell:
a: 59.666Å b: 88.425Å c: 95.12Å
α: 69.39° β: 89.84° γ: 77.07°
R-values:
R R work R free
0.208 0.206 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

History of DNA Helicases.
Brosh et al. (2020)
6 more

6 mentions without citation

New Insights Into DNA Helicases as Druggable Targets for Cancer Therapy.
Datta et al. (2018)
5 more