› 4cem

X-ray diffraction
2.6Å resolution

Crystal structure of the first MIF4G domain of human nonsense mediated decay factor UPF2

Released:
DOI: 10.2210/pdb4cem/pdb
PDB entry 4cem coloured by chain, front view.
PDB entry 4cem coloured by chain, side view.
PDB entry 4cem coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of the three MIF4G domains of nonsense-mediated decay factor UPF2.
Clerici M, Deniaud A, Boehm V, Gehring NH, Schaffitzel C, Cusack S
Nucleic Acids Res 42 2673-86 (2014)
PMID: 24271394

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190728 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulator of nonsense transcripts 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 370 amino acids
Theoretical weight: 42.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HAU5 (Residues: 121-486; Coverage: 29%)
Gene names: KIAA1408, RENT2, UPF2
Sequence domains: MIF4G domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P65
Unit cell:
a: 101.08Å b: 101.08Å c: 158.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.205 0.251
Expression system: Escherichia coli BL21(DE3)

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Citations

13 review citations

Nonsense-Mediated mRNA Decay: Degradation of Defective Transcripts Is Only Part of the Story.
He et al. (2015)
12 more

2 mentions without citation

No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors.
Mailliot et al. (2022)
1 more