› 4cfg

X-ray diffraction
2.8Å resolution

Structure of the TRIM25 coiled-coil

Released:
DOI: 10.2210/pdb4cfg/pdb
PDB entry 4cfg coloured by chain, front view.
PDB entry 4cfg coloured by chain, side view.
PDB entry 4cfg coloured by chain, top view.
Source organism: Homo sapiens
Entry author: James L

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
ATP + [ISG15] + [protein]-lysine = AMP + diphosphate + [protein]-N-ISGyllysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin/ISG15 ligase TRIM25 Chains: A, B
Molecule details ›
Chains: A, B
Length: 630 amino acids
Theoretical weight: 71.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14258 (Residues: 1-630; Coverage: 100%)
Gene names: EFP, RNF147, TRIM25, ZNF147
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND
Spacegroup: P21212
Unit cell:
a: 56.88Å b: 68.78Å c: 100.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.251 0.309
Expression system: Escherichia coli

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