› 4cfh

X-ray diffraction
3.24Å resolution

Structure of an active form of mammalian AMPK

Released:
DOI: 10.2210/pdb4cfh/pdb
PDB entry 4cfh coloured by chain, front view.
PDB entry 4cfh coloured by chain, side view.
PDB entry 4cfh coloured by chain, top view.
Source organisms:
Primary publication:
Structure of mammalian AMPK and its regulation by ADP.
Xiao B, Sanders MJ, Underwood E, Heath R, Mayer FV, Carmena D, Jing C, Walker PA, Eccleston JF, Haire LF, Saiu P, Howell SA, Aasland R, Martin SR, Carling D, Gamblin SJ
Nature 472 230-3 (2011)
PMID: 21399626

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [tau protein] = ADP + [tau protein] phosphate
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] = ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129098 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
5'-AMP-activated protein kinase catalytic subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 493 amino acids
Theoretical weight: 57.25 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P54645 (Residues: 13-483; Coverage: 84%)
Gene names: Ampk1, Prkaa1
Sequence domains:
5'-AMP-activated protein kinase subunit beta-2 Chain: B
Molecule details ›
Chain: B
Length: 87 amino acids
Theoretical weight: 10.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43741 (Residues: 187-272; Coverage: 32%)
Gene name: PRKAB2
Sequence domains: 5'-AMP-activated protein kinase beta subunit, interaction domain
5'-AMP-activated protein kinase catalytic subunit alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 27 amino acids
Theoretical weight: 3.06 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P54645 (Residues: 534-559; Coverage: 5%)
Gene names: Ampk1, Prkaa1
5'-AMP-activated protein kinase subunit gamma-1 Chain: E
Molecule details ›
Chain: E
Length: 330 amino acids
Theoretical weight: 37.43 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P80385 (Residues: 1-330; Coverage: 100%)
Gene name: Prkag1
Sequence domains: CBS domain

Ligands and Environments

2 bound ligands:
Ligand STU 1 x STU
Ligand AMP 2 x AMP
1 modified residue:
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P41212
Unit cell:
a: 133.917Å b: 133.917Å c: 141.896Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.268
Expression system: Escherichia coli

Quick links

Citations

206 review citations

AMPK: a nutrient and energy sensor that maintains energy homeostasis.
Hardie et al. (2012)
205 more

12 mentions without citation

Structure and Physiological Regulation of AMPK.
Yan et al. (2018)
11 more