› 4cgu

X-ray diffraction
2.11Å resolution

Full length Tah1 bound to yeast PIH1 and HSP90 peptide SRMEEVD

Released:
DOI: 10.2210/pdb4cgu/pdb
PDB entry 4cgu coloured by chain, front view.
PDB entry 4cgu coloured by chain, side view.
PDB entry 4cgu coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for phosphorylation-dependent recruitment of Tel2 to Hsp90 by Pih1.
Pal M, Morgan M, Phelps SE, Roe SM, Parry-Morris S, Downs JA, Polier S, Pearl LH, Prodromou C
Structure 22 805-18 (2014)
PMID: 24794838

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-139819 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
TPR repeat-containing protein associated with Hsp90 Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 12.78 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P25638 (Residues: 1-111; Coverage: 100%)
Gene names: TAH1, YCR060W, YCR60W
Sequence domains: Tetratricopeptide repeat
Protein interacting with Hsp90 1 Chain: B
Molecule details ›
Chain: B
Length: 158 amino acids
Theoretical weight: 18.11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38768 (Residues: 187-344; Coverage: 46%)
Gene names: NOP17, PIH1, YHR034C
Sequence domains: Fungal Pih1 CS domain
Structure domains: Immunoglobulin-like
Heat shock protein HSP 90-alpha Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 866 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P07900 (Residues: 726-732; Coverage: 1%)
Gene names: HSP90A, HSP90AA1, HSPC1, HSPCA

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2221
Unit cell:
a: 56.01Å b: 78.38Å c: 98.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.21
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 review citations

Hsp90: breaking the symmetry.
Mayer et al. (2015)
12 more

5 mentions without citation

The AAA+ proteins Pontin and Reptin enter adult age: from understanding their basic biology to the identification of selective inhibitors.
Matias et al. (2015)
4 more