› 4cgv

X-ray diffraction
2.54Å resolution

First TPR of Spaghetti (RPAP3) bound to HSP90 peptide SRMEEVD

Released:
DOI: 10.2210/pdb4cgv/pdb
PDB entry 4cgv coloured by chain, front view.
PDB entry 4cgv coloured by chain, side view.
PDB entry 4cgv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for phosphorylation-dependent recruitment of Tel2 to Hsp90 by Pih1.
Pal M, Morgan M, Phelps SE, Roe SM, Parry-Morris S, Downs JA, Polier S, Pearl LH, Prodromou C
Structure 22 805-18 (2014)
PMID: 24794838

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-139822 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA polymerase II-associated protein 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 136 amino acids
Theoretical weight: 15.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H6T3 (Residues: 120-255; Coverage: 21%)
Gene name: RPAP3
Sequence domains: Tetratricopeptide repeat
Structure domains: Tetratricopeptide repeat domain
Heat shock protein HSP 90-alpha Chains: E, F
Molecule details ›
Chains: E, F
Length: 7 amino acids
Theoretical weight: 866 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P07900 (Residues: 726-732; Coverage: 1%)
Gene names: HSP90A, HSP90AA1, HSPC1, HSPCA

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 52.05Å b: 59.12Å c: 65.86Å
α: 63.42° β: 67.5° γ: 82.43°
R-values:
R R work R free
0.242 0.24 0.267
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 review citations

Hsp90: breaking the symmetry.
Mayer et al. (2015)
12 more

2 mentions without citation

Cyclophilin40 isomerase activity is regulated by a temperature-dependent allosteric interaction with Hsp90.
Blackburn et al. (2015)
1 more