› 4cgz

X-ray diffraction
3.2Å resolution

Crystal structure of the Bloom's syndrome helicase BLM in complex with DNA

Released:
DOI: 10.2210/pdb4cgz/pdb
PDB entry 4cgz coloured by chain, front view.
PDB entry 4cgz coloured by chain, side view.
PDB entry 4cgz coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.
Newman JA, Savitsky P, Allerston CK, Bizard AH, Özer Ö, Sarlós K, Liu Y, Pardon E, Steyaert J, Hickson ID, Gileadi O
Nucleic Acids Res 43 5221-35 (2015)
PMID: 25901030

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117289 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
RecQ-like DNA helicase BLM Chain: A
Molecule details ›
Chain: A
Length: 665 amino acids
Theoretical weight: 75.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54132 (Residues: 636-1298; Coverage: 47%)
Gene names: BLM, RECQ2, RECQL3
Sequence domains:
Structure domains:
5'-D(*AP*GP*CP*GP*TP*CP*GP*AP*GP*AP*TP*CP)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.69 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*GP*AP*TP*CP*TP*CP*GP*AP*CP*GP*CP*TP*CP*DT *CP*CP*CP)-3' Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.1 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P42
Unit cell:
a: 149.53Å b: 149.53Å c: 64.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.229 0.259
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

History of DNA Helicases.
Brosh et al. (2020)
6 more

19 mentions without citation

New Insights Into DNA Helicases as Druggable Targets for Cancer Therapy.
Datta et al. (2018)
18 more