› 4chl

X-ray diffraction
2.61Å resolution

Human Ethylmalonic Encephalopathy Protein 1 (hETHE1)

Released:
DOI: 10.2210/pdb4chl/pdb
PDB entry 4chl coloured by chain, front view.
PDB entry 4chl coloured by chain, side view.
PDB entry 4chl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human persulfide dioxygenase: structural basis of ethylmalonic encephalopathy.
Pettinati I, Brem J, McDonough MA, Schofield CJ
Hum Mol Genet 24 2458-69 (2015)
PMID: 25596185

Function and Biology Details

Reaction catalysed: 
(1a) S-sulfanylglutathione + O(2) = glutathione S-dioxide + H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Persulfide dioxygenase ETHE1, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 26.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95571 (Residues: 21-254; Coverage: 92%)
Gene names: ETHE1, HSCO
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

1 bound ligand:
Ligand FE 2 x FE
1 modified residue:
Ligand CSD 2 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 73.94Å b: 124.93Å c: 63.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.223
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.
Bahr et al. (2021)
5 more

9 mentions without citation

Chemical Biology of H2S Signaling through Persulfidation.
Filipovic et al. (2018)
8 more