› 4ciz

X-ray diffraction
3.4Å resolution

Crystal structure of the complex of the Cellular Retinal Binding Protein with 9-cis-retinal

Released:
DOI: 10.2210/pdb4ciz/pdb
PDB entry 4ciz coloured by chain, front view.
PDB entry 4ciz coloured by chain, side view.
PDB entry 4ciz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human cellular retinaldehyde-binding protein has secondary thermal 9-cis-retinal isomerase activity.
Bolze CS, Helbling RE, Owen RL, Pearson AR, Pompidor G, Dworkowski F, Fuchs MR, Furrer J, Golczak M, Palczewski K, Cascella M, Stocker A
J Am Chem Soc 136 137-46 (2014)
PMID: 24328211

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinaldehyde-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 36.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P12271 (Residues: 1-317; Coverage: 100%)
Gene names: CRALBP, RLBP1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand RET 1 x RET
Ligand TLA 1 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P6522
Unit cell:
a: 70.556Å b: 70.556Å c: 299.747Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.234 0.265
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Shedding new light on the generation of the visual chromophore.
Palczewski et al. (2020)
8 other articles

1 mention without citation

Molecular Basis for Vitamin A Uptake and Storage in Vertebrates.
Chelstowska et al. (2016)