› 4cj6

X-ray diffraction
1.9Å resolution

Crystal structure of the complex of the Cellular Retinal Binding Protein Mutant R234W with 9-cis-retinal

Released:
DOI: 10.2210/pdb4cj6/pdb
PDB entry 4cj6 coloured by chain, front view.
PDB entry 4cj6 coloured by chain, side view.
PDB entry 4cj6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human cellular retinaldehyde-binding protein has secondary thermal 9-cis-retinal isomerase activity.
Bolze CS, Helbling RE, Owen RL, Pearson AR, Pompidor G, Dworkowski F, Fuchs MR, Furrer J, Golczak M, Palczewski K, Cascella M, Stocker A
J Am Chem Soc 136 137-46 (2014)
PMID: 24328211

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinaldehyde-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 36.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P12271 (Residues: 1-317; Coverage: 100%)
Gene names: CRALBP, RLBP1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 87.87Å b: 57.918Å c: 75.206Å
α: 90° β: 122.88° γ: 90°
R-values:
R R work R free
0.168 0.165 0.198
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Molecular Basis for Vitamin A Uptake and Storage in Vertebrates.
Chelstowska et al. (2016)
1 more

1 mention without citation

Enrichment patterns of intrinsic disorder in proteins.
Patil A. (2022)