› 4ck7

Electron Microscopy
9.2Å resolution

Pseudo-atomic model of microtubule-bound human kinesin-5 motor domain in presence of adp.alfx (NECK-LINKER IN ITS DISCONNECTED CONFORMATION, based on cryo-electron microscopy experiment

Released:
DOI: 10.2210/pdb4ck7/pdb
PDB entry 4ck7 coloured by chain, front view.
PDB entry 4ck7 coloured by chain, side view.
PDB entry 4ck7 coloured by chain, top view.
Source organisms:
Primary publication:
Comprehensive structural model of the mechanochemical cycle of a mitotic motor highlights molecular adaptations in the kinesin family.
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
Proc Natl Acad Sci U S A 111 1837-42 (2014)
PMID: 24449904
Related structures: EMD-2533

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-156613 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1D chain Chain: A
Molecule details ›
Chain: A
Length: 452 amino acids
Theoretical weight: 50.24 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2HJ86 (Residues: 1-452; Coverage: 100%)
Gene name: TUBA1D
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Kinesin-like protein KIF11 Chain: C
Molecule details ›
Chain: C
Length: 373 amino acids
Theoretical weight: 41.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P52732 (Residues: 1-367; Coverage: 35%)
Gene names: EG5, KIF11, KNSL1, TRIP5
Sequence domains: Kinesin motor domain

Ligands and Environments

6 bound ligands:
Ligand MG 2 x MG
Ligand GTP 1 x GTP
Ligand GDP 1 x GDP
Ligand TA1 1 x TA1
Ligand AF3 1 x AF3
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.2Å
Relevant EMDB volumes: EMD-2533
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Cryo-EM: A Unique Tool for the Visualization of Macromolecular Complexity.
Nogales et al. (2015)
6 more

1 mention without citation

Comprehensive structural model of the mechanochemical cycle of a mitotic motor highlights molecular adaptations in the kinesin family.
Goulet et al. (2014)