› 4cl1

X-ray diffraction
3.5Å resolution

The crystal structure of NS5A domain 1 from genotype 1a reveals new clues to the mechanism of action for dimeric HCV inhibitors

Released:
DOI: 10.2210/pdb4cl1/pdb
PDB entry 4cl1 coloured by chain, front view.
PDB entry 4cl1 coloured by chain, side view.
PDB entry 4cl1 coloured by chain, top view.
Source organism: Hepatitis C virus (isolate H77)
Primary publication:
The crystal structure of NS5A domain 1 from genotype 1a reveals new clues to the mechanism of action for dimeric HCV inhibitors.
Lambert SM, Langley DR, Garnett JA, Angell R, Hedgethorne K, Meanwell NA, Matthews SJ
Protein Sci 23 723-34 (2014)
PMID: 24639329

Function and Biology Details

Reactions catalysed: 
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125532 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NON-STRUCTURAL PROTEIN 5A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 177 amino acids
Theoretical weight: 19.83 KDa
Source organism: Hepatitis C virus (isolate H77)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K4KA16 (Residues: 2005-2174; Coverage: 6%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 100.23Å b: 101.76Å c: 149.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Hepatitis C virus RNA replication and assembly: living on the fat of the land.
Paul et al. (2014)
9 more

62 mentions without citation

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De Meester et al. (2022)
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