› 4cll

X-ray diffraction
1.7Å resolution

Crystal structure of human soluble Adenylyl Cyclase in complex with bicarbonate

Released:
DOI: 10.2210/pdb4cll/pdb
PDB entry 4cll coloured by chain, front view.
PDB entry 4cll coloured by chain, side view.
PDB entry 4cll coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human soluble adenylyl cyclase reveal mechanisms of catalysis and of its activation through bicarbonate.
Kleinboelting S, Diaz A, Moniot S, van den Heuvel J, Weyand M, Levin LR, Buck J, Steegborn C
Proc Natl Acad Sci U S A 111 3727-32 (2014)
PMID: 24567411

Function and Biology Details

Reaction catalysed: 
ATP = 3',5'-cyclic AMP + diphosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188670 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate cyclase type 10 Chain: A
Molecule details ›
Chain: A
Length: 475 amino acids
Theoretical weight: 54.27 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q96PN6 (Residues: 1-469; Coverage: 29%)
Gene names: ADCY10, SAC
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase

Ligands and Environments

3 bound ligands:
Ligand BCT 1 x BCT
Ligand EDO 10 x EDO
Ligand GOL 3 x GOL
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P63
Unit cell:
a: 99.83Å b: 99.83Å c: 98.06Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.162 0.198
Expression system: Trichoplusia ni

Quick links

Citations

26 review citations

Complex roles of cAMP-PKA-CREB signaling in cancer.
Zhang et al. (2020)
25 more

4 mentions without citation

Crystal structures of human soluble adenylyl cyclase reveal mechanisms of catalysis and of its activation through bicarbonate.
Kleinboelting et al. (2014)
3 more