› 4cqe

X-ray diffraction
2.3Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 31.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15056 (Residues: 448-723; Coverage: 36%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CQE 2 x CQE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 52.605Å b: 105.943Å c: 109.889Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

A patent review of RAF kinase inhibitors (2010-2018).
Man et al. (2019)
1 other article

4 mentions without citation

Allostery: Allosteric Cancer Drivers and Innovative Allosteric Drugs.
Nussinov et al. (2022)
3 more