› 4cxg

Electron Microscopy
8.7Å resolution

Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement

Released:
DOI: 10.2210/pdb4cxg/pdb
PDB entry 4cxg coloured by chain, front view.
PDB entry 4cxg coloured by chain, side view.
PDB entry 4cxg coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Regulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement.
Budkevich TV, Giesebrecht J, Behrmann E, Loerke J, Ramrath DJ, Mielke T, Ismer J, Hildebrand PW, Tung CS, Nierhaus KH, Sanbonmatsu KY, Spahn CM
Cell 158 121-31 (2014)
PMID: 24995983
Related structures: EMD-2623

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158599 (preferred)
Entry contents:
2 distinct polypeptide molecules
7 distinct RNA molecules
Macromolecules (9 distinct):
Elongation factor 1-alpha Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 48.73 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: Q9YAV0 (Residues: 1-437; Coverage: 100%)
Gene names: APE_1844, tuf
Sequence domains:
Small ribosomal subunit protein uS12 Chain: X
Molecule details ›
Chain: X
Length: 143 amino acids
Theoretical weight: 15.84 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P62266 (Residues: 1-143; Coverage: 100%)
Gene name: RPS23
Sequence domains: Ribosomal protein S12/S23
18S RRNA - H44 Chain: 1
Molecule details ›
Chain: 1
Length: 135 nucleotides
Theoretical weight: 43.64 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA
28S RRNA - H89 Chain: 2
Molecule details ›
Chain: 2
Length: 50 nucleotides
Theoretical weight: 15.97 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
TRANSFER RNA Chain: Y
Molecule details ›
Chain: Y
Length: 76 nucleotides
Theoretical weight: 24.69 KDa
Sequence domains: tRNA
18S RRNA - H5-H14 Chain: a
Molecule details ›
Chain: a
Length: 48 nucleotides
Theoretical weight: 15.42 KDa
18S RRNA - H8 Chain: b
Molecule details ›
Chain: b
Length: 17 nucleotides
Theoretical weight: 5.45 KDa
28S RRNA - H95 Chain: c
Molecule details ›
Chain: c
Length: 19 nucleotides
Theoretical weight: 6.14 KDa
Messenger RNA Chain: x
Molecule details ›
Chain: x
Length: 28 nucleotides
Theoretical weight: 8.53 KDa

Ligands and Environments

1 bound ligand:
Ligand PHE 1 x PHE
10 modified residues:
Ligand 2MG 1 x 2MG
Ligand H2U 2 x H2U
Ligand M2G 1 x M2G
Ligand OMC 1 x OMC
Ligand MIA 1 x MIA
Ligand 7MG 1 x 7MG
Ligand 5MC 1 x 5MC
Ligand 5MU 1 x 5MU
Ligand PSU 1 x PSU
Ligand 1MA 1 x 1MA

Experiments and Validation Details

Entry percentile scores
Resolution: 8.7Å
Relevant EMDB volumes: EMD-2623

Quick links

Citations

15 review citations

Organization and Regulation of Mitochondrial Protein Synthesis.
Ott et al. (2016)
14 more

2 mentions without citation

Regulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement.
Budkevich et al. (2014)
1 more