› 4cxn

X-ray diffraction
1.7Å resolution

Crystal structure of human insulin analogue (NMe-AlaB8)-insulin crystal form I

Released:
DOI: 10.2210/pdb4cxn/pdb
PDB entry 4cxn coloured by chain, front view.
PDB entry 4cxn coloured by chain, side view.
PDB entry 4cxn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insight into the structural and biological relevance of the T/R transition of the N-terminus of the B-chain in human insulin.
Kosinová L, Veverka V, Novotná P, Collinsová M, Urbanová M, Moody NR, Turkenburg JP, Jiráček J, Brzozowski AM, Žáková L
Biochemistry 53 3392-402 (2014)
PMID: 24819248

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.46 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MAA 1 x MAA

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: I213
Unit cell:
a: 79.17Å b: 79.17Å c: 79.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.209
Expression system: Not provided

Quick links

Citations

2 review citations

Progress in Simulation Studies of Insulin Structure and Function.
Gorai et al. (2022)
1 more