› 4cy2

X-ray diffraction
2Å resolution

Crystal structure of the KANSL1-WDR5-KANSL2 complex.

Released:
DOI: 10.2210/pdb4cy2/pdb
PDB entry 4cy2 coloured by chain, front view.
PDB entry 4cy2 coloured by chain, side view.
PDB entry 4cy2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of the KANSL1/WDR5/KANSL2 complex reveals that WDR5 is required for efficient assembly and chromatin targeting of the NSL complex.
Dias J, Van Nguyen N, Georgiev P, Gaub A, Brettschneider J, Cusack S, Kadlec J, Akhtar A
Genes Dev 28 929-42 (2014)
PMID: 24788516

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-158499 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61964 (Residues: 23-334; Coverage: 93%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
KAT8 regulatory NSL complex subunit 2 Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9H9L4 (Residues: 406-417; Coverage: 2%)
Gene names: C12orf41, KANSL2, NSL2
KAT8 regulatory NSL complex subunit 1 Chain: D
Molecule details ›
Chain: D
Length: 15 amino acids
Theoretical weight: 1.61 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q7Z3B3 (Residues: 585-598; Coverage: 1%)
Gene names: CENP-36, KANSL1, KIAA1267, MSL1V1, NSL1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21212
Unit cell:
a: 81.59Å b: 86.6Å c: 45.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.222
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

9 review citations

Moonlighting with WDR5: A Cellular Multitasker.
Guarnaccia et al. (2018)
8 more

4 mentions without citation

Interaction with WDR5 promotes target gene recognition and tumorigenesis by MYC.
Thomas et al. (2015)
3 more