› 4d0t

X-ray diffraction
2.45Å resolution

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-galactosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-alpha-D-galactosaminyl)-L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-164363 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polypeptide N-acetylgalactosaminyltransferase 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 571 amino acids
Theoretical weight: 64.82 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q10471 (Residues: 1-571; Coverage: 100%)
Gene name: GALNT2
Sequence domains:
Structure domains:
PEPTIDE Chains: P, Z
Molecule details ›
Chains: P, Z
Length: 8 amino acids
Theoretical weight: 789 Da
Source organism: synthetic construct

Ligands and Environments

5 bound ligands:
Ligand MN 6 x MN
Ligand EDO 10 x EDO
Ligand UD2 5 x UD2
Ligand UDP 1 x UDP
Ligand NGA 1 x NGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 117.217Å b: 122.372Å c: 249.724Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.241
Expression system: Komagataella pastoris

Quick links

Citations

8 review citations

The Biochemistry of O-GlcNAc Transferase: Which Functions Make It Essential in Mammalian Cells?
Levine et al. (2016)
7 more

11 mentions without citation

Generating orthogonal glycosyltransferase and nucleotide sugar pairs as next-generation glycobiology tools.
Cioce et al. (2021)
10 more