› 4d1o

X-ray diffraction
1.82Å resolution

Structure of human endothelial nitric oxide synthase heme domain with L-Arg bound

Released:
DOI: 10.2210/pdb4d1o/pdb
PDB entry 4d1o coloured by chain, front view.
PDB entry 4d1o coloured by chain, side view.
PDB entry 4d1o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of human constitutive nitric oxide synthases.
Li H, Jamal J, Plaza C, Pineda SH, Chreifi G, Jing Q, Cinelli MA, Silverman RB, Poulos TL
Acta Crystallogr D Biol Crystallogr 70 2667-74 (2014)
PMID: 25286850

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 440 amino acids
Theoretical weight: 49.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29474 (Residues: 41-480; Coverage: 37%)
Gene name: NOS3
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
7 bound ligands:
Ligand ARG 2 x ARG
Ligand ACT 2 x ACT
Ligand BTB 4 x BTB
Ligand GOL 3 x GOL
Ligand ZN 1 x ZN
Ligand H4B 2 x H4B
Ligand GD 2 x GD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 62.395Å b: 110.091Å c: 153.322Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.155 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Role of oxidative stress in the dysfunction of the placental endothelial nitric oxide synthase in preeclampsia.
Guerby et al. (2021)
2 more

9 mentions without citation

Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation
Tejero et al. (2019)
8 more