› 4d5n

Electron Microscopy
9Å resolution

Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state

Released:
DOI: 10.2210/pdb4d5n/pdb
PDB entry 4d5n coloured by chain, front view.
PDB entry 4d5n coloured by chain, side view.
PDB entry 4d5n coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM of ribosomal 80S complexes with termination factors reveals the translocated cricket paralysis virus IRES.
Muhs M, Hilal T, Mielke T, Skabkin MA, Sanbonmatsu KY, Pestova TV, Spahn CM
Mol Cell 57 422-32 (2015)
PMID: 25601755
Related structures: EMD-2810

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158632 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Eukaryotic peptide chain release factor subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 436 amino acids
Theoretical weight: 49.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62495 (Residues: 5-437; Coverage: 99%)
Gene names: ERF1, ETF1, RF1, SUP45L1
Sequence domains:
CRICKET PARALYSIS VIRUS IRES RNA Chain: X
Molecule details ›
Chain: X
Length: 201 nucleotides
Theoretical weight: 64.36 KDa
Source organism: Cricket paralysis virus
Expression system: Not provided
Sequence domains: Cripavirus internal ribosome entry site (IRES)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9Å
Relevant EMDB volumes: EMD-2810
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

20 review citations

RAN translation-What makes it run?
Green et al. (2016)
19 more

2 mentions without citation

Cryo-EM of ribosomal 80S complexes with termination factors reveals the translocated cricket paralysis virus IRES.
Muhs et al. (2015)
1 more