› 4dag

X-ray diffraction
3.39Å resolution

Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site

Released:
DOI: 10.2210/pdb4dag/pdb
PDB entry 4dag coloured by chain, front view.
PDB entry 4dag coloured by chain, side view.
PDB entry 4dag coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the human metapneumovirus fusion protein with neutralizing antibody identifies a pneumovirus antigenic site.
Wen X, Krause JC, Leser GP, Cox RG, Lamb RA, Williams JV, Crowe JE, Jardetzky TS
Nat Struct Mol Biol 19 461-3 (2012)
PMID: 22388735

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-124354 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Fusion glycoprotein F0 Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 44.95 KDa
Source organism: Human metapneumovirus
Expression system: Homo sapiens
UniProt:
  • Canonical: G3KCK8 (Residues: 18-432; Coverage: 77%)
Sequence domains: Fusion glycoprotein F0
Immunoglobulin heavy constant gamma 1 Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.11 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 1-98; Coverage: 25%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 22.54 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8N5F4 (Residues: 23-233; Coverage: 98%)
Gene name: IGL@
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P6322
Unit cell:
a: 258.96Å b: 258.96Å c: 167.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.261 0.26 0.29
Expression system: Homo sapiens

Quick links

Citations

9 review citations

Timing is everything: Fine-tuned molecular machines orchestrate paramyxovirus entry.
Bose et al. (2015)
8 more

14 mentions without citation

Max Bergmann lecture protein epitope mimetics in the age of structural vaccinology.
Robinson JA. (2013)
13 more