› 4dfw

X-ray diffraction
1.55Å resolution

Oxime-based Post Solid-phase Peptide Diversification: Identification of High Affinity Polo-like Kinase 1 (Plk1) Polo-box Domain Binding Peptides

Released:
DOI: 10.2210/pdb4dfw/pdb
PDB entry 4dfw coloured by chain, front view.
PDB entry 4dfw coloured by chain, side view.
PDB entry 4dfw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of high affinity polo-like kinase 1 (Plk1) polo-box domain binding peptides using oxime-based diversification.
Liu F, Park JE, Qian WJ, Lim D, Scharow A, Berg T, Yaffe MB, Lee KS, Burke TR
ACS Chem Biol 7 805-10 (2012)
PMID: 22292814

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156765 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PLK1 Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 27.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53350 (Residues: 367-603; Coverage: 39%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Structure domains: POLO box domain
Peptide Chain: D
Molecule details ›
Chain: D
Length: 7 amino acids
Theoretical weight: 807 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
2 modified residues:
Ligand 0LF 1 x 0LF
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 35.665Å b: 34.844Å c: 47.097Å
α: 76.44° β: 86.95° γ: 69.14°
R-values:
R R work R free
0.157 0.155 0.196
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Recent Advances and New Strategies in Targeting Plk1 for Anticancer Therapy.
Lee et al. (2015)
6 more

5 mentions without citation

Application of Post Solid-Phase Oxime Ligation to Fine-Tune Peptide-Protein Interactions.
Zhao et al. (2020)
4 more