4djc

X-ray diffraction
1.35Å resolution

1.35 A crystal structure of the NaV1.5 DIII-IV-Ca/CaM complex

Released:
Source organism: Homo sapiens
Primary publication:
Crystallographic basis for calcium regulation of sodium channels.
Proc Natl Acad Sci U S A 109 3558-63 (2012)
PMID: 22331908

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-143949 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 17.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Sodium channel protein type 5 subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 35 amino acids
Theoretical weight: 4.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14524 (Residues: 1491-1522; Coverage: 2%)
Gene name: SCN5A

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 28.38Å b: 72.72Å c: 97.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.176
Expression system: Escherichia coli