› 4djh

X-ray diffraction
2.9Å resolution

Structure of the human kappa opioid receptor in complex with JDTic

Released:
DOI: 10.2210/pdb4djh/pdb
PDB entry 4djh coloured by chain, front view.
PDB entry 4djh coloured by chain, side view.
PDB entry 4djh coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the human κ-opioid receptor in complex with JDTic.
Wu H, Wacker D, Mileni M, Katritch V, Han GW, Vardy E, Liu W, Thompson AA, Huang XP, Carroll FI, Mascarella SW, Westkaemper RB, Mosier PD, Roth BL, Cherezov V, Stevens RC
Nature 485 327-32 (2012)
PMID: 22437504

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132986 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Kappa-type opioid receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 480 amino acids
Theoretical weight: 54.29 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41145 (Residues: 43-261, 263-358; Coverage: 83%)
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
Gene names: E, OPRK, OPRK1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand JDC 2 x JDC
Ligand CIT 1 x CIT
Ligand OLC 2 x OLC
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 54.896Å b: 147.297Å c: 205.288Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.227 0.265
Expression system: Spodoptera frugiperda

Quick links

Citations

150 review citations

Molecular signatures of G-protein-coupled receptors.
Venkatakrishnan et al. (2013)
149 more

184 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
183 more