› 4dke

X-ray diffraction
3Å resolution

Crystal Structure of Human Interleukin-34 Bound to FAb1.1

Released:
DOI: 10.2210/pdb4dke/pdb
PDB entry 4dke coloured by chain, front view.
PDB entry 4dke coloured by chain, side view.
PDB entry 4dke coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R.
Ma X, Lin WY, Chen Y, Stawicki S, Mukhyala K, Wu Y, Martin F, Bazan JF, Starovasnik MA
Structure 20 676-87 (2012)
PMID: 22483114

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212423 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Interleukin-34 Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6ZMJ4 (Residues: 21-193; Coverage: 78%)
Gene names: C16orf77, IL34
Sequence domains: Interleukin 34
Structure domains: Interleukin-34
FAb1.1 Heavy Chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 230 amino acids
Theoretical weight: 24.43 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
FAb1.1 Light Chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 214 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN, BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 105.549Å b: 122.465Å c: 149.155Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.231 0.265
Expression systems:
  • Trichoplusia ni
  • Cricetulus griseus

Quick links

Citations

39 review citations

From Monocytes to M1/M2 Macrophages: Phenotypical vs. Functional Differentiation.
Italiani et al. (2014)
38 more

1 mention without citation

Structural basis of the broadly neutralizing anti-interferon-α antibody rontalizumab.
Maurer et al. (2015)