› 4dl7

X-ray diffraction
1.97Å resolution

Human DNA polymerase eta fails to extend primer 2 nucleotide after cisplatin crosslink (Pt-GG4).

Released:
DOI: 10.2210/pdb4dl7/pdb
PDB entry 4dl7 coloured by chain, front view.
PDB entry 4dl7 coloured by chain, side view.
PDB entry 4dl7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of human DNA polymerase η-mediated chemoresistance to cisplatin.
Zhao Y, Biertümpfel C, Gregory MT, Hua YJ, Hanaoka F, Yang W
Proc Natl Acad Sci U S A 109 7269-74 (2012)
PMID: 22529383

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117328 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase eta Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 48.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y253 (Residues: 1-432; Coverage: 61%)
Gene names: POLH, RAD30, RAD30A, XPV
Sequence domains:
Structure domains:
DNA (5'-D(*TP*AP*CP*TP*CP*GP*GP*TP*CP*AP*CP*T)-3') Chain: T
Molecule details ›
Chain: T
Length: 12 nucleotides
Theoretical weight: 3.61 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*AP*GP*TP*GP*AP*CP*CP*G)-3') Chain: P
Molecule details ›
Chain: P
Length: 9 nucleotides
Theoretical weight: 2.76 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand DZ4 1 x DZ4
Ligand CPT 1 x CPT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P61
Unit cell:
a: 99Å b: 99Å c: 81.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.192 0.214
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

21 review citations

Systems biology of cisplatin resistance: past, present and future.
Galluzzi et al. (2014)
20 more

4 mentions without citation

An overview of Y-Family DNA polymerases and a case study of human DNA polymerase η.
Yang W. (2014)
3 more